human normal liver cells thle2  (ATCC)

Journal: Scientific Reports

Article Title: ST8SIA6-AS1 contributes to hepatocellular carcinoma progression by targeting miR-142-3p/HMGA1 axis

doi: 10.1038/s41598-022-26643-8

Figure Lengend Snippet: ST8SIA6-AS1 upregulation in LIHC cells. ( A ) ST8SIA6-AS1 upregulated in LIHC tissues. **P < 0.001. ( B ) Upregulation of ST8SIA6-AS1 expression in LIHC cell lines (Hep3B, HCCLM3, Huh7, and SNU-182) comparison with normal liver epithelial cells THLE2. *P < 0.05, **P < 0.001 compared with THLE2. ( C ) RT-qPCR detection of GAPDH, and U6, and ST8SIA6-AS1, in nucleus and cell cytosol. ( D ) ST8SIA6-AS1 expression in HCCLM3 and in Huh7 cells treated with si-ST8SIA6-AS1. *P < 0.05, **P < 0.001 compared with blank. NC: negative control; blank: blank control; si-ST8SIA6-AS1: siRNA-ST8SIA6-AS1.

Article Snippet: LIHC cell lines (Hep3B, HCCLM3, Huh7, and SNU-182) and normal liver epithelial cells (THLE2) were provided by American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Comparison, Quantitative RT-PCR, Negative Control, Control

Journal: Scientific Reports

Article Title: ST8SIA6-AS1 contributes to hepatocellular carcinoma progression by targeting miR-142-3p/HMGA1 axis

doi: 10.1038/s41598-022-26643-8

Figure Lengend Snippet: ST8SIA6-AS1 sponged miR-142-3p in LIHC cells. ( A ) StarBase analysis prediction for binding sites between miR-142-3p and ST8SIA6-AS1. ( B ) Performance of Dual luciferase assessment in HCCLM3 and Huh7 cells. **P < 0.001 compared with NC. ( C ) The expression of miR-142-3p and ST8SIA6 AS1 are negatively correlated. ( D ) In LIHC cells (HCCLM3 and Huh7 cells), the expression of miR-142-3p was downregulate compared to normal cells (THLE2). **P < 0.001 compared with THLE2. ( E ) In HCCLM3 and Huh7 cells, miR-142-3p and ST8SIA6-AS1 expression was measured by RT-qPCR. ## P < 0.001 compared with si-lnc + anti-miR; **P < 0.001 compared with blank. WT: wild-type; Si-lnc: SiRNA-ST8SIA6-AS1, MUT: Mutant; anti-miR: miR-142-3p inhibitor, NC: negative control, and blank: blank control, Si-lnc + anti-miR (SiRNA-ST8SIA6-AS1 + miR-142-3p inhibitor).

Article Snippet: LIHC cell lines (Hep3B, HCCLM3, Huh7, and SNU-182) and normal liver epithelial cells (THLE2) were provided by American Type Culture Collection (ATCC, USA).

Techniques: Binding Assay, Luciferase, Expressing, Quantitative RT-PCR, Mutagenesis, Negative Control, Control

Journal: Scientific Reports

Article Title: ST8SIA6-AS1 contributes to hepatocellular carcinoma progression by targeting miR-142-3p/HMGA1 axis

doi: 10.1038/s41598-022-26643-8

Figure Lengend Snippet: MiR-142-3p repressed the level of HMGA1 in LIHC cells. ( A ) StarBase prediction of binding sequences between HMGA1 and miR-142-3p. ( B ) Dual luciferase assay was measured in HCCLM3 and Huh7 cells. **P < 0.001 comparison with NC. ( C ) The enrichment of miR-142-3p, ST8SIA6-AS1 and HMGA1 was determined by RT-qPCR. **P < 0.001 comparison with Bio-NC. ( D ) RT-qPCR used for determination of HMGA1 expression in LIHC tissues. **P < 0.001. ( E ) In LIHC tissues, there is a negative connection between miR-142-3p and HMGA1. ( F ) HMGA1 expression was measured using RT-qPCR in LIHC cells (HCCLM3 and Huh7 cells) and normal cells (THLE2). **P < 0.001 comparison with THLE2. ( G ) HMGA1 expression was measured using western blot in LIHC cells (HCCLM3 and Huh7 cells) and normal cells (THLE2). **P < 0.001 comparison with THLE2. ( H ) HMGA1 mRNA expression measurement in transfected Huh7 and HCCLM3 cells. ( I ) Expression of the HMGA1 protein in transfected Huh7 cells and HCCLM3 was estimated. **P < 0.001 comparison with blank; ## P < 0.001 comparison with si-lnc + anti-miR. NC: negative control; Si-lnc: SiRNA-ST8SIA6-AS1; anti-miR: miR-142-3p inhibitor; Si-lnc + anti-miR: SiRNA-ST8SIA6-AS1 + miR-142-3p inhibitor. ( J ) Measurement of HMGA1 expression in HCCLM3 and Huh7 cells was detected by western blot. ## P < 0.001 compared with si-HMGA1 + anti-miR; **P < 0.001 compared with blank. NC: negative control; blank: blank control, MUT: Mutant; WT: wild-type; Si-HMGA1 + anti-miR: SiRNA-HMGA1 + miR-142-3p inhibitor, and anti-miR: miR-142-3p inhibitor; Si-HMGA1: SiRNA-HMGA1.

Article Snippet: LIHC cell lines (Hep3B, HCCLM3, Huh7, and SNU-182) and normal liver epithelial cells (THLE2) were provided by American Type Culture Collection (ATCC, USA).

Techniques: Binding Assay, Luciferase, Comparison, Quantitative RT-PCR, Expressing, Western Blot, Transfection, Negative Control, Control, Mutagenesis